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COLGALT1 polyclonal antibody

货号: BS76860

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS76860

Product Name :COLGALT1 polyclonal antibody

Swiss-Prot :Q8NBJ5

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is one of two enzymes that transfers galactose moieties to hydroxylysine residues of collagen and mannose binding lectin. This gene is constitutively expressed and encodes a soluble protein that localizes to the endoplasmic reticulum.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :72KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :COLGALT1;GLT25D1

Immunogen :Recombinant fusion protein of human COLGALT1(NP_078932.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Mouse ovary, using COLGALT1 antibody at 1:1135 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of HeLa cells using COLGALT1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using COLGALT1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using COLGALT1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.