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UBE2E2 polyclonal antibody

货号: BS76787

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS76787

Product Name :UBE2E2 polyclonal antibody

Swiss-Prot :Q96LR5

Host :Rabbit

Reactivity :Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Ube2E2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It shares 96% identical amino acid sequence with Ube2E1 and Ube2E3 with the exception of the N-terminal extension that has little in common with the other two E2s. Ube2E2 interacts with ARA54 and RNF8. The RNF8/ Ube2E2 interaction is found to catalyze K48-linked poly-ubiquitination. Ube2E2 may play a role in ubiquitination and subsequent proteasomal degradation of the E3 Ub ligase Parkin.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :22KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :UBE2E2;UBCH8

Immunogen :Recombinant fusion protein of human UBE2E2(NP_689866.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using UBE2E2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.