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RARα polyclonal antibody

货号: BS7672

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS7672

Product Name :RARα polyclonal antibody

Swiss-Prot :P10276

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100

Background :This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :60KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NR1B1;RAR;RARA

Immunogen :Recombinant fusion protein of human RARα(NP_001019980.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using RARα antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

Immunofluorescence analysis of C6 cells using RARα Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using RARα Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.