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AQP2 polyclonal antibody

货号: BS76685

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS76685

Product Name :AQP2 polyclonal antibody

Swiss-Prot :P41181

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:200

Background :This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :26KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AQP2;AQP-CD;WCH-CD

Immunogen :A synthetic peptide of human AQP2(NP_000477.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of 293T cells, using AQP2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse kidney using AQP2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of rat kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse kidney cells using AQP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.