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USO1 polyclonal antibody

货号: BS76627

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS76627

Product Name :USO1 polyclonal antibody

Swiss-Prot :O60763

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100

Background :The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :108kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :USO1;P115;TAP;VDP

Immunogen :Recombinant fusion protein of human USO1(NP_003706.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using USO1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of HeLa cells using USO1 antibody at dilution of 1:200 . Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using USO1 antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of HeLa cells using USO1 Polyclonal Antibody at dilution of 1:50. Blue: DAPI for nuclear staining.