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PLA2G16 polyclonal antibody

货号: BS76600

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS76600

Product Name :PLA2G16 polyclonal antibody

Swiss-Prot :P53816

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Exhibits both phospholipase A1/2 and acyltransferase activities. Shows phospholipase A1 (PLA1 and A2 (PLA2 activity, catalyzing the calcium-independent release of fatty acids from the sn-1 or sn-2 position of glycerophospholipids. For most substrates, PLA1 activity is much higher than PLA2 activity. Shows O-acyltransferase activity,catalyzing the transfer of a fatty acyl group from glycerophospholipid to the hydroxyl group of lysophospholipid. Shows N-acyltransferase activity, catalyzing the calcium-independent transfer of a fatty acyl group at the sn-1 position of phosphatidylcholine (PC and other glycerophospholipids to the primary amine of phosphatidylethanolamine (PE, forming N-acylphosphatidylethanolamine (NAPE, which serves as precursor for N-acylethanolamines (NAEs. Exhibits high N-acyltransferase activity and low phospholipase A1/2 activity. Required for complete organelle rupture and degradation that occur during eye lens terminal differentiation, when fiber cells that compose the lens degrade all membrane-bound organelles in order to provide lens with transparency to allow the passage of light. Organelle membrane degradation is probably catalyzed by the phospholipase activity (By similarity.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :18kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PLA2G16;AdPLA;H-REV107;H-REV107-1;HRASLS3;HREV107;HREV107-1;HREV107-3;HRSL3

Immunogen :Recombinant fusion protein of human PLA2G16(NP_009000.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using PLA2G16 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 90s.