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PAPD5 polyclonal antibody

货号: BS76521

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS76521

Product Name :PAPD5 polyclonal antibody

Swiss-Prot :Q8NDF8

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3' poly(A tail creating a heterogeneous 3' poly(A tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation. Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro. Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1. Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion. Mediates 3' adenylation of the microRNA MIR21 followed by its 3'-to-5' trimming by the exoribonuclease PARN leading to degradation. Mediates 3' adenylation of H/ACA box snoRNAs (small nucleolar RNAs followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :90kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PAPD5;TRF4-2

Immunogen :Recombinant fusion protein of human PAPD5(NP_001035374.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using PAPD5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.