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UFM1 polyclonal antibody

货号: BS76503

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS76503

Product Name :UFM1 polyclonal antibody

Swiss-Prot :P61960

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 (UBE1DC1; MIM 610552) and E2-like conjugating enzyme UFC1 (MIM 610554) in a manner analogous to ubiquitylation (see UBE2M; MIM 603173) (Komatsu et al., 2004 [PubMed 15071506]).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :9KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :UFM1;BM-002;C13orf20

Immunogen :Recombinant fusion protein of human UFM1(NP_057701.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using UFM1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded mouse brain using UFM1 Rabbit pAb at dilution of 1:300 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of NIH/3T3 cells using UFM1 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using UFM1 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.