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DICER1 polyclonal antibody

货号: BS76480

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS76480

Product Name :DICER1 polyclonal antibody

Swiss-Prot :Q9UPY3

Host :Rabbit

Reactivity :Human

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :250KDaa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DICER1;DCR1;Dicer;Dicer1e;HERNA;K12H4.8-LIKE;MNG1;RMSE2

Immunogen :A synthetic peptide of human DICER1(NP_001258211.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of 293F, using DICER1 antibody at 1:994 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of U2OS cells using DICER1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.