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ANAPC11 polyclonal antibody

货号: BS76318

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS76318

Product Name :ANAPC11 polyclonal antibody

Swiss-Prot :Q9NYG5

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC, IF/ICC

Application_all :WB,1:200 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :Together with the cullin protein ANAPC2, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C, a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :12kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ANAPC11;APC11;Apc11p;HSPC214

Immunogen :Recombinant fusion protein of human ANAPC11(NP_001002245.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of mouse heart, using ANAPC11 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 120s.

Immunohistochemistry of paraffin-embedded human liver cancer using ANAPC11 Rabbit pAb at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse kidney using ANAPC11 Rabbit pAb at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of A-549 cells using ANAPC11 antibody at dilution of 1:300. Blue: DAPI for nuclear staining.