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NFIB polyclonal antibody

货号: BS76230

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS76230

Product Name :NFIB polyclonal antibody

Swiss-Prot :O00712

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IHC

Application_all :WB,1:200 - 1:2000|IHC,1:50 - 1:200

Background :Transcriptional activator of GFAP, essential for proper brain development. Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :47kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NFIB;CTF;HMGIC/NFIB;NF-I/B;NF1-B;NFI-B;NFI-RED;NFIB2;NFIB3

Immunogen :A synthetic peptide of human NFIB(NP_005587.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of HeLa cells, using NFIB antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 120s.

Immunohistochemistry of paraffin-embedded rat lung using NFIB Rabbit pAb at dilution of 1:250 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.