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SUMO2/3 polyclonal antibody

货号: BS76191

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS76191

Product Name :SUMO2/3 polyclonal antibody

Swiss-Prot :P61956

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :16kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SUMO2;HSMT3;SMT3B;SMT3H2;SUMO3;Smt3A

Immunogen :Recombinant fusion protein of human SUMO2/3(NP_008868.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using SUMO2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat lung using SUMO2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human gastric cancer using SUMO2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse lung using SUMO2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.