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SCNN1G polyclonal antibody

货号: BS76119

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS76119

Product Name :SCNN1G polyclonal antibody

Swiss-Prot :P51170

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:100 - 1:200|IF/ICC,1:50 - 1:200

Background :Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :74kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SCNN1G;BESC3;ENaCg;ENaCgamma;PHA1;SCNEG

Immunogen :Recombinant fusion protein of human SCNN1G(NP_001030.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using SCNN1G antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of human kidney cancer using SCNN1G antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse kidney using SCNN1G antibody at dilution of 1:100. Blue: DAPI for nuclear staining.