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DAPK3 polyclonal antibody

货号: BS76089

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS76089

Product Name :DAPK3 polyclonal antibody

Swiss-Prot :O43293

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100

Background :Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :55kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DAPK3;DLK;ZIP;ZIPK

Immunogen :A synthetic peptide of human DAPK3(NP_001339.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using DAPK3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using DAPK3 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human lung cancer using DAPK3 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human placenta using DAPK3 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.