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DDX49 polyclonal antibody

货号: BS76016

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS76016

Product Name :DDX49 polyclonal antibody

Swiss-Prot :Q9Y6V7

Host :Rabbit

Reactivity :Human

Applications :WB IHC FC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 FC: 1:10~1:50

Background :Enables RNA binding activity. Involved in positive regulation of cell growth and regulation of rRNA stability. Predicted to be located in nucleoplasm. Predicted to be active in nucleus.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :Refer to figures

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DDX49;R27090_2;Dbp8

Immunogen :Recombinant fusion protein of human DDX49(NP_061943.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Haptoglobin ) antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat liver using Haptoglobin ) antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human liver cancer using Haptoglobin ) antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.