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[KO Validated] MTH1 polyclonal antibody

货号: BS7594

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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No. :BS7594

Product Name :[KO Validated] MTH1 polyclonal antibody

Swiss-Prot :P36639

Host :Rabbit

Reactivity :Human

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:20 - 1:50

Background :Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :18kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NUDT1;MTH1

Immunogen :Recombinant fusion protein of human MTH1(NP_945192.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using MTH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Western blot analysis of extracts from normal and MTH1 knockout 293T cells, using MTH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug MTH1 antibody . Western blot was performed from the immunoprecipitate using MTH1 antibody at a dilution of 1:1000.