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SPRY3 polyclonal antibody

货号: BS75896

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS75896

Product Name :SPRY3 polyclonal antibody

Swiss-Prot :O43610

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Members of the Sprouty family (Sprouty 1-4) are inducible negative regulators of growth factors that act through tyrosine kinase receptors. Mammalian Sprouty homologs share a well-conserved cysteine-rich carboxy-terminal domain with their Drosophila counterparts. Sprouty proteins are cytoplasmic in unstimulated cells, but in cells stimulated by growth factors they anchor to the plasma membrane by palmitoylation. Sprouty 1 and 2 associate with caveolin-1 in perinuclear and vesicular structures and are phosphorylated on serine residues. Sprouty 2 can associate with c-Cbl, a downregulator of RTK signaling, and inhibit the activities of several growth factors. Unlike the widely expressed Sprouty members 1, 2 and 4, Sprouty 3 expression is restricted to adult brain and testis. Sprouty 4 is a target of the WNT/b-catenin signaling pathway in progenitor cells. In conclusion, members of Sprouty inhibit FGF and VEGF-mediated cell proliferation, suggesting that they may regulate angiogenesis in normal and disease processes.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :36kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SPRY3;spry-3

Immunogen :A synthetic peptide of human SPRY3(NP_005831.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of 293T cells, using SPRY3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.