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RUVBL1/TIP49A/PONTIN polyclonal antibody

货号: BS75638

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS75638

Product Name :RUVBL1/TIP49A/PONTIN polyclonal antibody

Swiss-Prot :Q9Y265

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :50kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RUVBL1;ECP-54;ECP54;INO80H;NMP 238;NMP238;PONTIN;Pontin52;RVB1;TIH1;TIP49;TIP49A

Immunogen :Recombinant fusion protein of human RUVBL1/TIP49A/PONTIN(NP_003698.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using RUVBL1/TIP49A/PONTIN antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of U2OS cells using RUVBL1/TIP49A/PONTIN antibody at dilution of 1:100. Blue: DAPI for nuclear staining.