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Emerin/EMD polyclonal antibody

货号: BS75527

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS75527

Product Name :Emerin/EMD polyclonal antibody

Swiss-Prot :P50402

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :35kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EMD;EDMD;LEMD5;STA;emerin

Immunogen :Recombinant fusion protein of human Emerin/Emerin/EMD(NP_000108.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Emerin/Emerin/EMD antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human gastric cancer using Emerin/Emerin/EMD antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human uterine cancer using Emerin/Emerin/EMD antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of U-2 OS cells using Emerin/Emerin/EMD antibody at dilution of 1:100. Blue: DAPI for nuclear staining.