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EIF4E3 polyclonal antibody

货号: BS75506

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS75506

Product Name :EIF4E3 polyclonal antibody

Swiss-Prot :Q8N5X7

Host :Rabbit

Reactivity :Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :EIF4E3 belongs to the EIF4E family of translational initiation factors that interact with the 5-prime cap structure of mRNA and recruit mRNA to the ribosome (Joshi et al., 2004 [PubMed 15153109]).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :24kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EIF4E3;eIF-4E3;eIF4E-3

Immunogen :Recombinant fusion protein of human EIF4E3(NP_775495.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using EIF4E3 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.