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Prohibitin polyclonal antibody

货号: BS75358

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS75358

Product Name :Prohibitin polyclonal antibody

Swiss-Prot :P35232

Host :Rabbit

Reactivity :Human, Mouse, Rat, Monkey

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :30kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HEL-215;HEL-S-54e;PHB1;PHB;Prohibitin

Immunogen :Recombinant fusion protein of human Prohibitin(NP_002625.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Prohibitin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human breast cancer using Prohibitin Rabbit pAb at dilution of 1:500 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human lung cancer using Prohibitin Rabbit pAb at dilution of 1:500 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.