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Clusterin alpha chain polyclonal antibody

货号: BS75346

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS75346

Product Name :Clusterin alpha chain polyclonal antibody

Swiss-Prot :P10909

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :38kDa/57kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CLU;AAG4;APO-J;APOJ;CLI;CLU1;CLU2;KUB1;NA1/NA2;SGP-2;SGP2;SP-40;TRPM-2;TRPM2;clusterin

Immunogen :Recombinant fusion protein of human Clusterin alpha chain(NP_976084.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Clusterin alpha chain antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of U2OS cells using Clusterin alpha chain antibody . Blue: DAPI for nuclear staining.