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HSP47/SERPINH1 polyclonal antibody

货号: BS75345

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS75345

Product Name :HSP47/SERPINH1 polyclonal antibody

Swiss-Prot :P50454

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :46KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SERPINH1;AsTP3;CBP1;CBP2;HSP47;OI10;PIG14;PPROM;RA-A47;SERPINH2;gp46;serpin H1

Immunogen :Recombinant fusion protein of human HSP47/HSP47/SERPINH1(NP_001226.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using HSP47/SERPINH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of NIH/3T3 cells using HSP47/SERPINH1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.