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Cation-independent M6PR (IGF2R) polyclonal antibody

货号: BS75131

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS75131

Product Name :Cation-independent M6PR (IGF2R) polyclonal antibody

Swiss-Prot :P11717

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :274KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :IGF2R;CD222;CI-M6PR;CIMPR;M6P-R;M6P/IGF2R;MPR 300;MPR1;MPR300;MPRI

Immunogen :Recombinant fusion protein of human Cation-independent M6PR (Cation-independent M6PR (IGF2R))(NP_000867.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of HepG2 cells, using Cation-independent M6PR ) antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunofluorescence analysis of C6 cells using Cation-independent M6PR ) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Cation-independent M6PR ) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Cation-independent M6PR ) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.