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TRIM25 polyclonal antibody

货号: BS75101

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS75101

Product Name :TRIM25 polyclonal antibody

Swiss-Prot :Q14258

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :68kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TRIM25;EFP;RNF147;Z147;ZNF147

Immunogen :Recombinant fusion protein of human TRIM25(NP_005073.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using TRIM25 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of L929 cells using TRIM25 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug TRIM25 antibody . Western blot was performed from the immunoprecipitate using TRIM25 antibody at a dilution of 1:1000.