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YY1 polyclonal antibody

货号: BS75095

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS75095

Product Name :YY1 polyclonal antibody

Swiss-Prot :P25490

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, ChIP

Application_all :WB,1:1000 - 1:2000|ChIP,1:50 - 1:200

Background :YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :65KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DELTA;INO80S;NF-E1;UCRBP;YIN-YANG-1;YY1

Immunogen :Recombinant fusion protein of human YY1(NP_003394.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using YY1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Chromatin immunoprecipitation analysis of extracts of 293T cells, using YY1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.