产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

NSUN3 polyclonal antibody

货号: BS75075

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS75075

Product Name :NSUN3 polyclonal antibody

Swiss-Prot :Q9H649

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C at position 34 of mt-tRNA(Met. mt-tRNA(Met methylation at cytosine(34 takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f(5c at this position. mt-tRNA(Met containing the f(5c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :38kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NSUN3;MST077;MSTP077

Immunogen :Recombinant fusion protein of human NSUN3(NP_071355.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using NSUN3 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.