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ZNF281 polyclonal antibody

货号: BS74954

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74954

Product Name :ZNF281 polyclonal antibody

Swiss-Prot :Q9Y2X9

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:1000 - 1:3000|IHC,1:50 - 1:200

Background :Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity. Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :110kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ZNF281;ZBP-99;ZNP-99

Immunogen :Recombinant fusion protein of human ZNF281(NP_036614.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of mouse spleen, using ZNF281 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.