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[KO Validated] HP1 alpha/CBX5 polyclonal antibody

货号: BS74916

价格：￥ 1540

货期：现货（1-2个工作日）

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No. :BS74916

Product Name :[KO Validated] HP1 alpha/CBX5 polyclonal antibody

Swiss-Prot :P45973

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :22kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CBX5;HEL25;HP1;HP1A

Immunogen :Recombinant fusion protein of human HP1 alpha/HP1 alpha/CBX5(NP_001120794.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using HP1 alpha/HP1 alpha/CBX5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Western blot analysis of extracts from normal and HP1 alpha/HP1 alpha/CBX5 Rabbit pAb knockout 293T cells, using HP1 alpha/HP1 alpha/CBX5 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of U2OS cells using HP1 alpha/HP1 alpha/CBX5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.