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UBE2A polyclonal antibody

货号: BS74897

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS74897

Product Name :UBE2A polyclonal antibody

Swiss-Prot :P49459

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :17kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :UBE2A;HHR6A;MRXS30;MRXSN;RAD6A;UBC2

Immunogen :Recombinant fusion protein of human UBE2A(NP_003327.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of mouse kidney, using UBE2A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of U2OS cells using UBE2A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.