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FGFR2 polyclonal antibody

货号: BS74863

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS74863

Product Name :FGFR2 polyclonal antibody

Swiss-Prot :P21802

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :145kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BBDS;BEK;BFR-1;CD332;CEK3;CFD1;ECT1;JWS;K-SAM;KGFR;TK14;TK25;FGFR2

Immunogen :Recombinant fusion protein of human FGFR2(NP_001138390.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of MCF7 cells, using FGFR2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunofluorescence analysis of HeLa cells using FGFR2 antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using FGFR2 antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using FGFR2 antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

Oligodendrocyte-astrocyte crosstalk in Parkinson’s disease mediates neuronal ferroptosis via the FGF signaling pathway

Journalnpj Parkinsons Disease

ApplicationIF

IF6.7

SpeciesMouse

PMID40410211