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PALLD polyclonal antibody

货号: BS74832

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS74832

Product Name :PALLD polyclonal antibody

Swiss-Prot :Q8WX93

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IP, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IP,50 - 1:200|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a cytoskeletal protein that is required for organizing the actin cytoskeleton. The protein is a component of actin-containing microfilaments, and it is involved in the control of cell shape, adhesion, and contraction. Polymorphisms in this gene are associated with a susceptibility to pancreatic cancer type 1, and also with a risk for myocardial infarction. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :150kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PALLD;CGI-151;CGI151;MYN;PNCA1;SIH002;palladin

Immunogen :Recombinant fusion protein of human PALLD(NP_001159582.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using PALLD antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat ovary using PALLD Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human colon carcinoma using PALLD Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of HeLa cells using PALLD Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.