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CDH11 polyclonal antibody

货号: BS74774

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS74774

Product Name :CDH11 polyclonal antibody

Swiss-Prot :P55287

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:1000|IF/ICC,1:50 - 1:200

Background :This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :110kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CDH11;CAD11;CDHOB;OB;OSF-4

Immunogen :Recombinant fusion protein of human CDH11(P55287).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using CDH11 antibody at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.

Immunofluorescence analysis of L929 cells using CDH11 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.