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Cytokeratin 1 (KRT1) polyclonal antibody

货号: BS74697

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74697

Product Name :Cytokeratin 1 (KRT1) polyclonal antibody

Swiss-Prot :P04264

Host :Rabbit

Reactivity :Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:1000

Background :The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :66kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :KRT1;CK1;EHK;EHK1;EPPK;K1;KRT1A;NEPPK;keratin 1

Immunogen :Recombinant fusion protein of human Cytokeratin 1 (Cytokeratin 1 (KRT1))(NP_006112.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Cytokeratin 1 ) antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.