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PHGDH polyclonal antibody

货号: BS74511

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74511

Product Name :PHGDH polyclonal antibody

Swiss-Prot :O43175

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :57KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PHGDH;3-PGDH;3PGDH;HEL-S-113;NLS;NLS1;PDG;PGAD;PGD;PGDH;PHGDHD;SERA

Immunogen :Recombinant fusion protein of human PHGDH(NP_006614.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using PHGDH antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of HeLa cells using PHGDH Rabbit pAb at dilution of 1:300 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using PHGDH Rabbit pAb at dilution of 1:300 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using PHGDH Rabbit pAb at dilution of 1:300 . Blue: DAPI for nuclear staining.