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KRT3 polyclonal antibody

货号: BS74371

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS74371

Product Name :KRT3 polyclonal antibody

Swiss-Prot :P12035

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :65kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :KRT3;CK3;K3;keratin 3

Immunogen :Recombinant fusion protein of human KRT3(NP_476429.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of H460 cells, using KRT3 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of A431 cells using KRT3 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.