产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

LEF1 polyclonal antibody

货号: BS74307

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74307

Product Name :LEF1 polyclonal antibody

Swiss-Prot :Q9UJU2

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IP,IF/ICC

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :25-58kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LEF1;LEF-1;TCF10;TCF1ALPHA;TCF7L3

Immunogen :Recombinant fusion protein of human LEF1(NP_057353.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using LEF1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of mouse thymus cells using LEF1 Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat thymus cells using LEF1 Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug LEF1 antibody . Western blot was performed from the immunoprecipitate using LEF1 antibody at a dilution of 1:1000.