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TSC2 polyclonal antibody

货号: BS74267

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS74267

Product Name :TSC2 polyclonal antibody

Swiss-Prot :P49815

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:200 - 1:500|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100

Background :Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :200KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LAM;PPP1R160;TSC4;TSC2;Tuberin;tuberin

Immunogen :Recombinant fusion protein of human TSC2(NP_000539.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using TSC2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat brain using TSC2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human thyroid cancer using TSC2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse brain using TSC2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.