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Ret polyclonal antibody

货号: BS74232

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74232

Product Name :Ret polyclonal antibody

Swiss-Prot :P07949

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB,IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :150KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RET;CDHF12;CDHR16;HSCR1;MEN2A;MEN2B;MTC1;PTC;RET-ELE1;RET51

Immunogen :A synthetic peptide of human Ret(NP_066124.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat brain, using Ret antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.

Immunofluorescence analysis of Neuro-2a cells using Ret Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of SH-SY5Y cells using Ret Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.