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Phospho-BRD4-S1070 polyclonal antibody

货号: BS74213

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74213

Product Name :Phospho-BRD4-S1070 polyclonal antibody

Swiss-Prot :O60885

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :250KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BRD4; CAP; HUNK1; HUNKI; MCAP; bromodomain containing 4

Immunogen :A synthetic phosphorylated peptide around S1070 of human BRD4(NP_490597.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of HeLa cells, using Phospho-BRD4-S1070 antibody at 1:1000 dilution.HeLa cells were treated by Calyculin A at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.