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Phospho-PPIG-S376 polyclonal antibody

货号: BS74188

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74188

Product Name :Phospho-PPIG-S376 polyclonal antibody

Swiss-Prot :Q13427

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :110kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PPIG;CARS-Cyp;CYP;SCAF10;SRCyp

Immunogen :A synthetic phosphorylated peptide around S376 of human PPIG(NP_004783.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of various cell lines, using Phospho-PPIG-S376 antibody at 1:1000 dilution. 293T cell lysate were treated by CIP at 37℃ for 1 hour. C2C12 cell lysate were treated by CIP at 37℃ for 1 hour. PC-12 cell lysate were treated by CIP at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 90s.