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Phospho-eEF2-T56 polyclonal antibody

货号: BS74185

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74185

Product Name :Phospho-eEF2-T56 polyclonal antibody

Swiss-Prot :P13639

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :105kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EEF2;EEF-2;EF-2;EF2;SCA26

Immunogen :A synthetic phosphorylated peptide around T56 of human Phospho-eEF2-T56(NP_001952.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of various cell lines, using Phospho-eEF2-T56 antibody at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin for 30 minutes after serum-starvation overnight. C6 cells were treated by Forskolin for 1 hour after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 5s.