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Phospho-LAT-Y171 polyclonal antibody

货号: BS74179

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74179

Product Name :Phospho-LAT-Y171 polyclonal antibody

Swiss-Prot :O43561

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :38kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LAT;LAT1;pp36;IMD52

Immunogen :A synthetic phosphorylated peptide around Y171 of human LAT(NP_001014987.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of Jurkat cells, using Phospho-LAT-Y171 antibody at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide for 2 minutes after serum-starvation overnight or treated by CD-3 for 2 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit .
Exposure time: 5s.