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Phospho-PAK2-S20 polyclonal antibody

货号: BS74178

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74178

Product Name :Phospho-PAK2-S20 polyclonal antibody

Swiss-Prot :Q13177

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :58kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PAK2;PAK65;PAKgamma

Immunogen :A synthetic phosphorylated peptide around S20 of human PAK2(NP_002568.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of HeLa cells, using Phospho-PAK2-S20 antibody at 1:2000 dilution. HeLa cells were treated by Calyculin A for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 5s.

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PAK2-S20 pAb at 1:2000 dilution or PAK2 antibody .NIH/3T3 cells were treated by Calyculin A for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 5s.