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Phospho-EphA3-Y779 polyclonal antibody

货号: BS74165

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74165

Product Name :Phospho-EphA3-Y779 polyclonal antibody

Swiss-Prot :P29320

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :110kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EK4;ETK;HEK;ETK1;HEK4;TYRO4;EPHA3

Immunogen :A synthetic phosphorylated peptide around Y779 of human EphA3(NP_005224.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of 293T and Hela cells, using Phospho-EphA3-Y779 antibody at 1:2000 dilution. 293T cells were treated by Pervanadate for 30 minutes after serum-starvation overnight.HeLa cells were treated by Pervanadate for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 1s.