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Phospho-GSK3α-S21 polyclonal antibody

货号: BS74159

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74159

Product Name :Phospho-GSK3α-S21 polyclonal antibody

Swiss-Prot :P49840

Host :Rabbit

Reactivity :Human

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:100

Background :This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :51kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GSK3 alpha;GSK3A

Immunogen :A synthetic phosphorylated peptide around S21 of human GSK3α(NP_063937.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of 293 and HeLa cells, using Phospho-GSK3α-S21 antibody at 1:1000 dilution. 293 cells were treated by Insulin for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Western blot analysis of extracts of 293 cells, using Phospho-GSK3α-S21 antibody at 1:1000 dilution or GSK3α antibody .293 cells were treated by Insulin for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-GSK3α-S21 pAb . Western blot was performed from the immunoprecipitate using Phospho-GSK3α-S21 pAb at a dilution of 1:1000. 293T cells were treated by Insulin at 37℃ for 10 minutes after serum-starvation overnight.