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Phospho-cdc25C-S216 polyclonal antibody

货号: BS74158

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74158

Product Name :Phospho-cdc25C-S216 polyclonal antibody

Swiss-Prot :P30307

Host :Rabbit

Reactivity :Human, Rat

Applications :WB,IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :53kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CDC25C;CDC25;PPP1R60

Immunogen :A synthetic phosphorylated peptide around S216 of human cdc25C(NP_001781.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of HT-29 cells, using Phospho-cdc25C-S216 antibody .
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.