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Phospho-TrkA-Y674/675 + TrkB-Y706/707 polyclonal antibody

货号: BS74152

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74152

Product Name :Phospho-TrkA-Y674/675 + TrkB-Y706/707 polyclonal antibody

Swiss-Prot :P04629/Q16620

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :140KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NTRK1/NTRK2

Immunogen :A synthetic phosphorylated peptide around Y674 & Y675 of human TrkANTRK1(NP_001012331.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of HeLa cells, using Phospho-TrkA-Y674/675 + TrkB-Y706/707 antibody at 1:500 dilution.HeLa cells were treated by Pervanadate at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.