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Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 polyclonal antibody

货号: BS74139

价格：￥ 1680

货期：现货（1-2个工作日）

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No. :BS74139

Product Name :Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 polyclonal antibody

Swiss-Prot :P56524/Q9UQL6/Q9UKV0

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:100

Background :Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :125kDa/140kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HDAC4/HDAC5/HDAC9

Immunogen :A phospho specific peptide corresponding to residues surrounding S246 of humanHDAC4

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts from 293 cells untreated or treated with EGF using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Antibody .
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Immunohistochemistry of paraffin-embedded human placenta using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat brain using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.