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Phospho-ERK1-Y204 polyclonal antibody

货号: BS74136

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74136

Product Name :Phospho-ERK1-Y204 polyclonal antibody

Swiss-Prot :P27361

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:100 - 1:200|IP,1:50 - 1:100

Background :The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :42KDa/44KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ERK-1;ERK1;ERT2;HS44KDAP;HUMKER1A;P44ERK1;P44MAPK;PRKM3;p44-ERK1;p44-MAPK;MAPK3

Immunogen :A synthetic phosphorylated peptide around Y204 of human ERK1(NP_002737.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ERK1-Y204 antibody at 1:1000 dilution.NIH/3T3 cells were treated by PMA/TPA at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat liver using Phospho-ERK1-Y204 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human uterus using Phospho-ERK1-Y204 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse heart using Phospho-ERK1-Y204 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.